Here you can find a list of studies and literature related to the use of Leucocyte- and Platelet-Rich Fibrin (L-PRF) in tissue regeneration These studies cover a range of topics, including clinical trials, systematic reviews and meta-analyses, as well as basic science research into the mechanisms and applications of L-PRF.
The following is a summary of key findings and themes from the available literature:
Clinical trials and studies: several clinical trials and proof-of-concept studies have been conducted to evaluate the efficacy of L-PRF in various applications, such as increasing the width of the cornified mucosa around implants, bone augmentation procedures, socket management, ridge preservation, periodontal regeneration and sinus floor elevation.
Systematic reviews and meta-analyses: These studies compile and analyze existing evidence from multiple clinical trials and provide comprehensive assessments of the regenerative potential of L-PRF in a variety of contexts, including intrabony defects, furcation defects, periodontal plastic surgery, sinus floor elevation, alveolar ridge preservation and implant therapy.
Mechanisms and properties of L-PRF: Basic scientific research has investigated the biological mechanisms underlying the regenerative effects of L-PRF, including fibrin architecture, leukocyte content, growth factor release kinetics and antimicrobial properties. These studies contribute to the understanding of how L-PRF promotes tissue healing and regeneration.
Comparative studies: Some studies compare different platelet concentrates, such as platelet-rich plasma (PRP), platelet-rich fibrin (PRF) and platelet gel, to assess their relative efficacy and benefits in wound healing and tissue regeneration.
Clinical applications beyond dentistry: Although many studies focus on dental and oral applications of L-PRF, some are also exploring its potential in orthopedic surgery, sports medicine, wound care and other medical fields, highlighting the versatility and broad utility of L-PRF in tissue regeneration.
Overall, you can see the literature as L-PRF shows promise and regenerative therapy for various clinical scenarios.
It offers benefits such as improved wound healing, reduced inflammation and enhanced tissue regeneration compared with traditional treatment methods.
Dental – Maxillo Facial - Periodontal
L-PRF membranes for increasing the width of keratinized mucosa around implants: A split-mouth, randomized, controlled pilot clinical trial.Temmerman Andy, Cleeren Gert-Jan, Castro Ana & Quirynen Marc. Journal of Periodontal Research (2018) Submitted.
L-PRF block for bone augmentation procedure: a proof-of-concept study. Cortellini Simone, Temmerman Andy, Ana Castro, Vandessel Jeroen, Jacobs Reinhilde, Wim Teughels & Quirynen Marc.Journal of Clinical Periodontology (2018) Accepted.
Regenerative potential of Leucocyte- and Platelet Rich Fibrin (L-PRF). Part A: intrabony defects, furcation defects, and periodontal plastic surgery. A systematic review and meta-analysis. Castro Ana, Meschi Nastaran, Temmerman Andy, Pinto Nelson, Lambrechts Paul, Teughels Wim, Quirynen Marc.Journal of Clinical Periodontology (2017) 44: 67-82.`
Regenerative potential of Leucocyte- and Platelet Rich Fibrin (L-PRF). Part B: sinus floor elevation, alveolar ridge preservation, and implant therapy. A systematic review and meta-analysis. Castro Ana, Meschi Nastaran, Temmerman Andy, Pinto Nelson, Lambrechts Paul, Teughels Wim, Quirynen Marc. Journal of Clinical Periodontology (2017) 44: 225-234.
The use of leucocyte and platelet-rich fibrin in socket management and ridge preservation: a split-mouth, randomized, controlled clinical trial. Temmerman Andy, Vandessel Jeroen, Castro Ana, Jacobs Reinhilde, Teughels Wim, Pinto Nelson, Quirynen Marc. Journal of Clinical Periodontology (2016) 43: 990-999.
L-PRF in Periodontal Regeneration Nelson Pinto, Andy Temmerman, Simone Cortellini, Ana Castro, Wim Teughels & Marc Quirynen.Newman: Carranza’s Clinical Periodontology 13e edition – Elsevier To be published in 2018.
Dallari, D., Savarino, L., Stagni, C., Cenni, E., Cenacchi, A., Fornasari, P. M., Albisinni, U., Rimondi, E., Baldini, N. & Giunti, A. (2007).
Enhanced tibial osteotomy healing with use of bone grafts supplemented with platelet gel or platelet gel and bone marrow stromal cells. Journal of Bone and Joint Surgery. American volume,89, 2413-2420.
Weefselregeneratie door middel van L-PRF: ‘van mythe tot realiteit’. Andy Temmerman, Iris De Coster, Ana Castro Sarda, Nelson Pinto, Wim Teughels en Marc Quirynen Het tandheelkundig jaar 2017. Bohn Stafleu van Loghum ISBN 978-90-368-1029-6.
Del Fabbro, M., Bortolin, M., Taschieri, S. & Weinstein, R. (2011). Is platelet concentrate advantageous for the surgical treatment of periodontal diseases? A systematic review and meta-analysis. Journal of Periodontology,82, 1100-1011.
Dohan Ehrenfest, D. M., Choukroun, J., Diss, A., Dohan, S. L., Dohan, A. J., Mouhyi, J. & Gogly, B. (2006). Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surgery, Oral Medidine, Oral Pathology, Oral Radiology and Endodontics,101, e45-50.
Frykberg, R. G., Driver, V. R., Carman, D., Lucero, B., Borris-Hale, C., Fylling, C. P., Rappl, L. M. & Clausen, P. A. (2010). Chronic wounds treated with a physiologically relevant concentration of platelet-rich plasma gel: a prospective case series. Ostomy Wound Manage,56, 36-44.
Inchingolo, F., Tatullo, M., Marrelli, M., Inchingolo, A. M., Inchingolo, A. D., Dipalma, G., Flace, P., Girolamo, F., Tarullo, A., Laino, L., Sabatini, R., Abbinante, A. & Cagiano, R. (2012). Regenerative surgery performed with platelet-rich plasma used in sinus lift elevation before dental implant surgery: an useful aid in healing and regeneration of bone tissue. European Review for Medical and Pharmacological Sciences,16, 1222-1226.
Intini, G. (2009). The use of platelet-rich plasma in bone reconstruction therapy. Biomaterials,30, 4956-4966.
Marrelli, M. & Tatullo, M. (2013). Influence of PRF in the healing of bone and gingival tissues. Clinical and histological evaluations. European Review of Medical and Pharmacological Sciences,17, 1958-1962.
Simonpieri, A., Choukroun, J., Del Corso, M., Sammartino, G. & Dohan Ehrenfest, D. M. (2011). Simultaneous sinus-lift and implantation using microthreaded implants and leukocyte- and platelet-rich fibrin as sole grafting material: a six year experience. Implant Dentistry, 20, 2-12.
L-PRF membranes for increasing the width of keratinized mucosa around implants: A split-mouth, randomized, controlled pilot clinical trial. Temmerman Andy, Cleeren Gert-Jan, Castro Ana & Quirynen Marc. Journal of Periodontal Research (2018) Submitted.
L-PRF block for bone augmentation procedure: a proof-of-concept study. Cortellini Simone, Temmerman Andy, Ana Castro, Vandessel Jeroen, Jacobs Reinhilde, Wim Teughels & Quirynen Marc. Journal of Clinical Periodontology (2018) Accepted.
Regenerative potential of Leucocyte- and Platelet Rich Fibrin (L-PRF). Part A: intrabony defects, furcation defects, and periodontal plastic surgery. A systematic review and meta-analysis. Castro Ana, Meschi Nastaran, Temmerman Andy, Pinto Nelson, Lambrechts Paul, Teughels Wim, Quirynen Marc. journal of Clinical Periodontology (2017) 44: 67-82.
Regenerative potential of Leucocyte- and Platelet Rich Fibrin (L-PRF). Part B: sinus floor elevation, alveolar ridge preservation, and implant therapy. A systematic review and meta-analysis. Castro Ana, Meschi Nastaran, Temmerman Andy, Pinto Nelson, Lambrechts Paul, Teughels Wim, Quirynen Marc. Journal of Clinical Periodontology (2017) 44: 225-234.
The use of leucocyte and platelet-rich fibrin in socket management and ridge preservation: a split-mouth, randomized, controlled clinical trial. Temmerman Andy, Vandessel Jeroen, Castro Ana, Jacobs Reinhilde, Teughels Wim, Pinto Nelson, Quirynen Marc. Journal of Clinical Periodontology (2016) 43: 990-999.
L-PRF in Periodontal Regeneration Nelson Pinto, Andy Temmerman, Simone Cortellini, Ana Castro, Wim Teughels & Marc Quirynen.Newman: Carranza’s Clinical Periodontology 13e edition – Elsevier
Weefselregeneratie door middel van L-PRF: ‘van mythe tot realiteit’. Andy Temmerman, Iris De Coster, Ana Castro Sarda, Nelson Pinto, Wim Teughels en Marc Quirynen Het tandheelkundig jaar 2017. Bohn Stafleu van Loghum ISBN 978-90-368-1029-
Del Fabbro, M., Bortolin, M., Taschieri, S. & Weinstein, R. (2011). Is platelet concentrate advantageous for the surgical treatment of periodontal diseases? A systematic review and meta-analysis. Journal of Periodontology,82, 1100-1011.
Dohan Ehrenfest, D. M., Choukroun, J., Diss, A., Dohan, S. L., Dohan, A. J., Mouhyi, J. & Gogly, B. (2006). Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics,101, e45-50.
TECHNICAL
The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors and fibrin architecture of a Leukocyte- and Platelet-Rich Fibrin (L-PRF) clot and membrane. David M. Dohan Ehrenfest, Byung-Soo Kang, Marco Del Corso, Mauricio Nally, Marc Quirynen, Hom-Lay Wang and Nelson R. Pinto.
Current misperceptions about protocols and systemsNew Biomaterials and Regenerative Medicine Strategies in Periodontology, Oral Surgery, Esthetic and Implant Dentistry 2016
WOUND HEALINGAdamson, R. (2009). Role of macrophages in normal wound healing: an overview. Journal of Wound Care, 18, 349-351.
Ahmed, T. A., Dare, E. V. & Hincke, M. (2008). Fibrin: a versatile scaffold for tissue engineering applications. Tissue Engineering Part B: Reviews, 14, 199-215.
Anitua, E., Sanchez, M., Orive, G. & Andia, I. (2007). The potential impact of the preparation rich in growth factors (PRGF) in different medical fields. Biomaterials, 28, 4551-4560.
Barrick, B., Campbell, E. J. & Owen, C. A. (1999). Leukocyte proteinases in wound healing: roles in physiologic and pathologic processes. Wound Repair and Regeneration, 7, 410-422.
Barrientos, S., Stojadinovic, O., Golinko, M. S., Brem, H. & Tomic-Canic, M. (2008). Growth factors and cytokines in wound healing. Wound Repair and Regeneration, 16, 585-601.
Bielecki, T., Dohan Ehrenfest, D. M., Everts, P. A. & Wiczkowski, A. (2012). The role of leukocytes from L-PRP/L-PRF in wound healing and immune defense: new perspectives. Current Pharmaceutical Biotechnology, 13, 1153-1162.
Lara, F. J., Serrano, A. M., Moreno, J. U., Carmona, J. H., Marquez, M. F., Perez, L. R., Del Rey Moreno, A. & Munoz, H. O. (2015). Platelet-rich fibrin sealant as a treatment for complex perianal fistulas: a multicentre study. Journal of Gastrointestinal Surgery, 19, 360-368.
Lingen, M. W. (2001). Role of leukocytes and endothelial cells in the development of angiogenesis in inflammation and wound healing. Archives of Pathololgy and Laboratory Medicine, 125, 67-71.
Martin, P. & Leibovich, S. J. (2005). Inflammatory cells during wound repair: the good, the bad and the ugly. Trends in Cell Biology, 15, 599-607.
Reksodiputro, M., Widodo, D., Bashiruddin, J., Siregar, N. & Malik, S. (2014). PRFM enhance wound healing process in skin graft. Facial Plastic Surgery, 30, 670-675.
Adamson, R. (2009). Role of macrophages in normal wound healing: an overview. Journal of Wound Care, 18, 349-351.
Ahmed, T. A., Dare, E. V. & Hincke, M. (2008). Fibrin: a versatile scaffold for tissue engineering applications. Tissue Engineering Part B: Reviews, 14, 199-215.
Anitua, E., Sanchez, M., Orive, G. & Andia, I. (2007). The potential impact of the preparation rich in growth factors (PRGF) in different medical fields. Biomaterials, 28, 4551-4560.
Barrick, B., Campbell, E. J. & Owen, C. A. (1999). Leukocyte proteinases in wound healing: roles in physiologic and pathologic processes. Wound Repair and Regeneration, 7, 410-422.
Barrientos, S., Stojadinovic, O., Golinko, M. S., Brem, H. & Tomic-Canic, M. (2008). Growth factors and cytokines in wound healing. Wound Repair and Regeneration, 16, 585-601.
Schultz, G. S. & Wysocki, A. (2009). Interactions between extracellular matrix and growth factors in wound healing. Wound Repair Regeneration, 17, 153-162.
Tsirogianni, A. K., Moutsopoulos, N. M. & Moutsopoulos, H. M. (2006). Wound healing: immunological aspects. Injury, 37 Suppl 1, S5-12.
BIOLOGICAL - BIOTECHNOLOGY
Blair, P. & Flaumenhaft, M. D. (2009). Platelet α-granules: basic biology and clinical correlates. Blood reviews, 23, 177-189.
Boswell, S. G., Cole, B. J., Sundman, E. A., Karas, V. & Fortier, L. A. (2012). Platelet-rich plasma: a milieu of bioactive factors. Arthroscopy, 28, 429-439.
Cieslik-Bielecka, A., Dohan Ehrenfest, D. M., Lubkowska, A. & Bielecki, T. (2012). Microbicidal properties of Leukocyte- and Platelet-Rich Plasma/Fibrin (L-PRP/L-PRF): new perspectives. Journal of Biological Regulator and Homeostatic Agents, 26, 43S-52S
Dohan Ehrenfest, D. M., Bielecki, T., Jimbo, R., Barbe, G., Del Corso, M., Inchingolo, F. & Sammartino, G. (2012). Do the fibrin architecture and leukocyte content influence the growth factor release of platelet concentrates? An evidence-based answer comparing a pure platelet-rich plasma (P-PRP) gel and a leukocyte- and platelet-rich fibrin (L-PRF). Current Pharmaceutical Biotechnology, 13, 1145-1452.
Dohan Ehrenfest, D. M., De Peppo, G. M., Doglioli, P. & Sammartino, G. (2009). Slow release of growth factors and thrombospondin-1 in Choukroun's platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies. Growth Factors, 27, 63-69.
Dohan Ehrenfest, D. M., Del Corso, M., Diss, A., Mouhyi, J. & Charrier, J. B. (2010). Three-dimensional architecture and cell composition of a Choukroun's platelet-rich fibrin clot and membrane. Journal of Periodontology, 81, 546-555.
Eming, S. A., Hammerschmidt, M., Krieg, T. & Roers, A. (2009). Interrelation of immunity and tissue repair or regeneration. Seminars in Cell and Developmental Biology, 20, 517-527.
Giannini, S., Cielo, A., Bonanome, L., Rastelli, C., Derla, C., Corpaci, F. & Falisi, G. (2015). Comparison between PRP, PRGF and PRF: lights and shadows in three similar but different protocols. European Review of Medical and Pharmacological Sciencies, 19, 927-930.
Blair, P. & Flaumenhaft, M. D. (2009). Platelet α-granules: basic biology and clinical correlates. Blood reviews, 23, 177-189.
Boswell, S. G., Cole, B. J., Sundman, E. A., Karas, V. & Fortier, L. A. (2012). Platelet-rich plasma: a milieu of bioactive factors. Arthroscopy, 28, 429-439.
Cieslik-Bielecka, A., Dohan Ehrenfest, D. M., Lubkowska, A. & Bielecki, T. (2012). Microbicidal properties of Leukocyte- and Platelet-Rich Plasma/Fibrin (L-PRP/L-PRF): new perspectives. Journal of Biological Regulator and Homeostatic Agents, 26, 43S-52S.
ORTHOPEDICS - SPORTS
Castillo, T. N., Pouliot, M. A., Kim, H. J. & Dragoo, J. L. (2011). Comparison of growth factor and platelet concentration from commercial platelet-rich plasma separation systems. American Journal of Sports Medicine, 39, 266-271.
Dohan Ehrenfest, D. M., Andia, I., Zumstein, M. A., Zhang, C. Q., Pinto, N. R. & Bielecki, T. (2014). Classification of platelet concentrates (Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications and perspectives. Muscles Ligaments and Tendons Journal, 4, 3-9.
Dragoo, J. L., Braun, H. J., Durham, J. L., Ridley, B. A., Odegaard, J. I., Luong, R. & Arnoczky, S. P. (2012). Comparison of the acute inflammatory response of two commercial platelet-rich plasma systems in healthy rabbit tendons. Am Journal of Sports Medicine, 40, 1274-1281.
Foster, T. E., Puskas, B. L., Mandelbaum, B. R., Gerhardt, M. B. & Rodeo, S. A. (2009). Platelet-rich plasma: from basic science to clinical applications. American Journal of Sports Medicine, 37, 2259-2272.
Hall, M. P., Band, P. A., Meislin, R. J., Jazrawi, L. M. & Cardone, D. A. (2009). Platelet-rich plasma: current concepts and application in sports medicine. Journal of the American Academy of Orthopaedic Surgeons, 17, 602-608.
Lopez-Vidriero, E., Goulding, K. A., Simon, D. A., Sanchez, M. & Johnson, D. H. (2010). The use of platelet-rich plasma in arthroscopy and sports medicine: optimizing the healing environment. Arthroscopy, 26, 269-278.
Mazzocca, A. D., Mccarthy, M. B., Chowaniec, D. M., Cote, M. P., Romeo, A. A., Bradley, J. P., Arciero, R. A. & Beitzel, K. (2012). Platelet-rich plasma differs according to preparation method and human variability. Journal fo Bone and Joint Surgery. American volume, 94, 308-316.
Mishra, A., Harmon, K., Woodall, J. & Vieira, A. (2012). Sports medicine applications of platelet rich plasma. Curr Pharm Biotechnol, 13, 1185-1195.
Moojen, D. J., Everts, P. A., Schure, R. M., Overdevest, E. P., Van Zundert, A., Knape, J. T., Castelein, R. M., Creemers, L. B. & Dhert, W. J. (2008). Antimicrobial activity of platelet-leukocyte gel against Staphylococcus aureus. Journal of Orthopaedic Research, 26, 404-410.
Pape, H. C., Marcucio, R., Humphrey, C., Colnot, C., Knobe, M. & Harvey, E. J. (2010). Trauma-induced inflammation and fracture healing. Journal of Orthopaedic Trauma, 24, 522-525
Randelli, P., Arrigoni, P., Ragone, V., Aliprandi, A. & Cabitza, P. (2011). Platelet rich plasma in arthroscopic rotator cuff repair: a prospective RCT study, 2-year follow-up. Journal of Shoulder and Elbow Surgery, 20, 518-528.
Marrelli, M., Paduano, F. & Tatullo, M. (2015). Human periapical cyst-mesenchymal stem cells differentiate into neuronal cells. J Dent Res, 94, 843-852.
Rodeo, S. A., Delos, D., Weber, A., Ju, X., Cunningham, M. E., Fortier, L. & Maher, S. (2010). What's new in orthopaedic research. Journal of Bone and Joint Surgery. American volume, 92, 2491-2501.
Sanchez, M., Anitua, E., Azofra, J., Andia, I., Padilla, S. & Mujika, I. (2007). Comparison of surgically repaired Achilles tendon tears using platelet-rich fibrin matrices. American Journal of Sports Medicine, 35, 245-251.
Schnabel, L. V., Lynch M. E., van der Meulen, M. C., Yeager, A. E., Kornatowksi, M. A. & Nixon, A. J. (2009). Mesenchymal stem cells and insulin-like growth factor-I-gene-enhanced mesenchymal stem cells improve structural aspects of healing in equine flexor digitorum superficialis tendons. Journal of Orthopaedics Research, 27, 1392-1398.
Tang, Y. Q., Yeaman, M. R. & Selsted, M. E. (2002). Antimicrobial peptides from human platelets. Infections and Immunity, 70, 6524-6533.
Tsirogianni, A. K., Moutsopoulos, N. M. & Moutsopoulos, H. M. (2006). Wound healing: immunological aspects. Injury, 37 Suppl 1, S5-12.
Visser, L. C., Arnoczky, S. P., Caballero, O. & Egerbacher, M. (2010). Platelet-rich fibrin constructs elute higher concentrations of transforming growth factor-beta-1 and increase tendon cell proliferation over time when compared to blood clots: a comparative in vitro analysis. Veterinary Surgery, 39, 811-817.
Zimmermann, R., Arnold, D., Strasser, E., Ringwald, J., Schlegel, A., Wiltfang, J. & Eckstein, R. (2003). Sample preparation technique and white cell content influence the detectable levels of growth factors in platelet concentrates. Vox Sanguinis, 85, 283-289.
Zumstein, M. A., Bielecki, T. & Dohan Ehrenfest, D. M. (2011). The future of platelet concentrates in sport medicine: Platelet-Rich Plasma, Platelet-Rich Fibrin, and the impact of scaffolds and cells on teh lon-term delivery of growth factors. Operative techniques in Sports Medicine, 19, 190-197.
Zumstein, M. A., Berger, S., Schober, M., Boileau, P., Nyffeler, R. W., Horn, M. & Dahinden, C. A. (2012). Leukocyte- and platelet-rich fibrin (L-PRF) for long-term delivery of growth factor in rotator cuff repair: review, preliminary results and future directions. Current Pharmaceutical Biotechnology, 13, 1196-1206.
Dallari, D., Savarino, L., Stagni, C., Cenni, E., Cenacchi, A., Fornasari, P. M., Albisinni, U., Rimondi, E., Baldini, N. & Giunti, A. (2007). Enhanced tibial osteotomy healing with use of bone grafts supplemented with platelet gel or platelet gel and bone marrow stromal cells. Journal of Bone and Joint Surgery. American volume, 89, 2413-2420.
Current misperceptions about protocols and systems - New Biomaterials and Regenerative Medicine Strategies in Periodontology, Oral Surgery, Esthetic and Implant Dentistry 2016
Dohan Ehrenfest, D. M., Bielecki, T., Jimbo, R., Barbe, G., Del Corso, M., Inchingolo, F. & Sammartino, G. (2012). Do the fibrin architecture and leukocyte content influence the growth factor release of platelet concentrates? An evidence-based answer comparing a pure platelet-rich plasma (P-PRP) gel and a leukocyte- and platelet-rich fibrin (L-PRF). Current Pharmaceutical Biotechnology, 13, 1145-1452.
Dohan Ehrenfest, D. M., De Peppo, G. M., Doglioli, P. & Sammartino, G. (2009). Slow release of growth factors and thrombospondin-1 in Choukroun's platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies. Growth Factors, 27, 63-69.
Dohan Ehrenfest, D. M., Del Corso, M., Diss, A., Mouhyi, J. & Charrier, J. B. (2010). Three-dimensional architecture and cell composition of a Choukroun's platelet-rich fibrin clot and membrane. Journal of Periodontology, 81, 546-555.
Dragoo, J. L., Braun, H. J., Durham, J. L., Ridley, B. A., Odegaard, J. I., Luong, R. & Arnoczky, S. P. (2012). Comparison of the acute inflammatory response of two commercial platelet-rich plasma systems in healthy rabbit tendons. Am Journal of Sports Medicine, 40, 1274-1281.
Eming, S. A., Hammerschmidt, M., Krieg, T. & Roers, A. (2009). Interrelation of immunity and tissue repair or regeneration. Seminars in Cell and Developmental Biology, 20, 517-527.
Foster, T. E., Puskas, B. L., Mandelbaum, B. R., Gerhardt, M. B. & Rodeo, S. A. (2009). Platelet-rich plasma: from basic science to clinical applications. American Journal of Sports Medicine, 37, 2259-2272.
Frykberg, R. G., Driver, V. R., Carman, D., Lucero, B., Borris-Hale, C., Fylling, C. P., Rappl, L. M. & Clausen, P. A. (2010). Chronic wounds treated with a physiologically relevant concentration of platelet-rich plasma gel: a prospective case series. Ostomy Wound Manage, 56, 36-44.
Giannini, S., Cielo, A., Bonanome, L., Rastelli, C., Derla, C., Corpaci, F. & Falisi, G. (2015). Comparison between PRP, PRGF and PRF: lights and shadows in three similar but different protocols. European Review of Medical and Pharmacological Sciencies, 19, 927-930.
Hall, M. P., Band, P. A., Meislin, R. J., Jazrawi, L. M. & Cardone, D. A. (2009). Platelet-rich plasma: current concepts and application in sports medicine. Journal of the American Academy of Orthopaedic Surgeons, 17, 602-608.
Inchingolo, F., Tatullo, M., Marrelli, M., Inchingolo, A. M., Inchingolo, A. D., Dipalma, G., Flace, P., Girolamo, F., Tarullo, A., Laino, L., Sabatini, R., Abbinante, A. & Cagiano, R. (2012). Regenerative surgery performed with platelet-rich plasma used in sinus lift elevation before dental implant surgery: an useful aid in healing and regeneration of bone tissue. European Review for Medical and Pharmacological Sciences, 16, 1222-1226.
Intini, G. (2009). The use of platelet-rich plasma in bone reconstruction therapy. Biomaterials, 30, 4956-4966.
Lara, F. J., Serrano, A. M., Moreno, J. U., Carmona, J. H., Marquez, M. F., Perez, L. R., Del Rey Moreno, A. & Munoz, H. O. (2015). Platelet-rich fibrin sealant as a treatment for complex perianal fistulas: a multicentre study. Journal of Gastrointestinal Surgery, 19, 360-368.
Lingen, M. W. (2001). Role of leukocytes and endothelial cells in the development of angiogenesis in inflammation and wound healing. Archives of Pathololgy and Laboratory Medicine, 125, 67-71.
Lopez-Vidriero, E., Goulding, K. A., Simon, D. A., Sanchez, M. & Johnson, D. H. (2010). The use of platelet-rich plasma in arthroscopy and sports medicine: optimizing the healing environment. Arthroscopy, 26, 269-278.
Marrelli, M. & Tatullo, M. (2013). Influence of PRF in the healing of bone and gingival tissues. Clinical and histological evaluations. European Review of Medical and Pharmacological Sciences, 17, 1958-1962.
Marrelli, M., Paduano, F. & Tatullo, M. (2015). Human periapical cyst-mesenchymal stem cells differentiate into neuronal cells. J Dent Res, 94, 843-852.
Martin, P. & Leibovich, S. J. (2005). Inflammatory cells during wound repair: the good, the bad and the ugly. Trends in Cell Biology, 15, 599-607.
Mazzocca, A. D., Mccarthy, M. B., Chowaniec, D. M., Cote, M. P., Romeo, A. A., Bradley, J. P., Arciero, R. A. & Beitzel, K. (2012). Platelet-rich plasma differs according to preparation method and human variability. Journal fo Bone and Joint Surgery. American volume, 94, 308-316.
Mishra, A., Harmon, K., Woodall, J. & Vieira, A. (2012). Sports medicine applications of platelet rich plasma. Curr Pharm Biotechnol, 13, 1185-1195.
Schnabel, L. V., Lynch M. E., van der Meulen, M. C., Yeager, A. E., Kornatowksi, M. A. & Nixon, A. J. (2009). Mesenchymal stem cells and insulin-like growth factor-I-gene-enhanced mesenchymal stem cells improve structural aspects of healing in equine flexor digitorum superficialis tendons. Journal of Orthopaedics Research, 27, 1392-1398.
Tang, Y. Q., Yeaman, M. R. & Selsted, M. E. (2002). Antimicrobial peptides from human platelets. Infections and Immunity, 70, 6524-6533.
Visser, L. C., Arnoczky, S. P., Caballero, O. & Egerbacher, M. (2010). Platelet-rich fibrin constructs elute higher concentrations of transforming growth factor-beta-1 and increase tendon cell proliferation over time when compared to blood clots: a comparative in vitro analysis. Veterinary Surgery, 39, 811-817.
Zimmermann, R., Arnold, D., Strasser, E., Ringwald, J., Schlegel, A., Wiltfang, J. & Eckstein, R. (2003). Sample preparation technique and white cell content influence the detectable levels of growth factors in platelet concentrates. Vox Sanguinis, 85, 283-289.