APAG and the blood phase separator
How does this work?
When a wound is sustained, platelets are the first cells to be activated. They aggregate to stop bleeding and then release substances (growth factors, cytokines, chemokines) to naturally activate the healing process. In wounds where complete healing does not occur, the mechanism of release of these substances by platelets may be interrupted.
Healing process:
The wound is first filled by a blood clot, whose mesh of fibrin contains red blood cells and leukocytes; in the surrounding tissue, rapid inflammation occurs with exudate formation, fibrin deposits and infiltration of neutrophils. Inflammation is then followed by the healing process starting at the edges of the wound where the original tissue is in contact with the blood clot Macrophages perform an important function as they enter the clot and remove decomposing red blood cells, fibrin and cellular deposits.
A.P.A.G.
It is simple and quick to prepare application that can be used in the doctor's office.
The doctor takes a small amount of peripheral blood from the patient with sterile vacuum tubes without exogenous substances, using the separator of blood components "Medifuge" after about 14 min (separation occurred) we notice three components.
Following fractions are withdrawn from the blood tube by fraction using a different sterile syringe:
- Interface 0.7 ml CD34+.
- Interface 2 ml PRP
- Remaining 2 ml PPP
Take 1 ml PPP in a 2.5 ml syringe and insert it into the antistatic and antimagnetic heating denaturaliser APAG; you will get the denaturalisation of albumin after 10/12 minutes.
Then leave the contents of the syringe for 10 minutes in absence of heat and absence of direct light and you will get APAG.
Mix 1 ml A.P.A.G. with 0.3 ml CD34+, 0.2 ml PRP, 0.2 ml ascorbic acid, using 2 sterile syringes with a 3-way connection for 30 seconds.
Now the gel can be applied over the entire wound, where afterwards an autologous membrane is also subsequently used to cover.
The autologous membrane is always obtained from the same blood sample and the same separation process: the collected blood is placed in a different sterile vacuum tube from the previous one.
With PV200R blood tubes, we can obtain solid-phase separation, from which a membrane is formed; strong, elastic and adhesive measuring about 1.5 x 2.5 cm.
The membrane stimulates the growth of epithelium on the wound. With no pressure dressing, the membrane guides and helps overcome the inflammatory process.
Applications:
A.P.A.G. - PRP - CD34+, in 10 days - 45/50-year-old patient - "3 applications".
A.P.A.G. - PRP - CD34+, in 10 days - 51/61-year-old patient - "3/4 applications".
A.P.A.G. - PRP - CD34+, in 10 days - 65/80-year-old patient - "5 applications".
Growth of granulation tissue in 7 days = 50% contrite wound - volume reduction of 90%.
Matrix for definitive closure with excellent nutritional support performed 2 times a week for 8 weeks to 12 weeks.
A process of new cells and differentiation into specialised structures of the tissue itself arises from the healing tissue (which forms an important barrier against infection).
The final outcome of the healing process depends on the relative contribution of repair and regeneration; if the wound undergoes microbial contamination during this process, it is much less likely to heal which prolongs the stage of infection.
Guided healing method:
Most treatments offered only serve to stem the wounds and are rarely effective deep inside where healing is most important.
A.P.A.G. + PRP + CD34+: is a semi-fluid gel containing autologous platelets from the patient. It takes on the shape and size of the wound. This stimulates the formation of new tissue throughout the wound, three-dimensionally and helps reduce the volume in a short time, reducing pain and the risk of infection and promoting healing.
Closed wounds:
- With the use of A.P.A.G. and autologous growth factors PRP and CD34+, the healing rate is 81.3% in wounds smaller than 7 cm
- diabetic ulcer recovered in 6.2 weeks.
Without the use of autologous growth factors, healing is 42.1% in wounds smaller than 7 cm
- diabetic ulcer recovered in 32 weeks.
- Reduction of baseline in less than one month - healing 2/3 months maximum of recurrent ulcer one year - chronic
- Effective in diabetic foot of 1st and 2nd degree.
Membrane effects:
Contraindications:
Do not perform in patients under chemotherapy, malignancy in the wound area, Haemoglobin 10.5 g / o / l, Serum albumin 2.5 g / or / l, Platelets 100x10 g / l, Wounds declared clinically infectious. Application in Aesthetic-Cosmetic and Surgical, Odonto Dental, and Maxillo - Facial, etc. will follow.
