International Journal of Molecular SciencesArticle

Analysis of CGF Biomolecules, Structure and Cell Population:Characterization of the Stemness Features of CGF Cells and Osteogenic Potential 

Eleonora Stanca 1,*, Nadia Calabriso 2 , Laura Giannotti 1, Paola Nitti 3,4 , Fabrizio Damiano 1 ,Benedetta Di Chiara Stanca 1, Maria Annunziata Carluccio 2 , Giuseppe Egidio De Benedetto 4 ,Christian Demitri 3, Andrea Palermo 5, Franco Ferrante 6 , Luisa Siculella 1,* and Alessio Rochira 1,* https://www.mdpi.com/1422-0067/22/16/8867

Plast Reconstr Surg .2008 Nov;122(5):1352-1360. doi:10.1097/PRS.0b013e3181882046.

Proliferation-promoting effect of platelet-rich plasma on human adipose-derived stem cells and human dermal fibroblasts Natsuko Kakudo ¹, Tatsuya Minakata, Toshihito Mitsui, Satoshi Kushida, Frederik Zefanya Notodihardjo, Kenji KusumotoAffiliations expand PMID: 18971718 DOI: 10.1097/PRS.0b013e3181882046

Comparative Study J Pediatr Orthop . 2007 Sep;27(6):629-34.doi: 10.1097/BPO.0b013e318093f523.

Distraction osteogenesis of the lower extremity in patients with achondroplasia/hypochondroplasia treated with transplantation of culture-expanded bone marrow cells and platelet-rich plasma Hiroshi Kitoh ¹, Takahiko Kitakoji, Hiroki Tsuchiya, Mitsuyasu Katoh, Naoki IshiguroAffiliations expand PMID: 17717461 DOI: 10.1097/BPO.0b013e318093f523

J Med Eng . 2013;2013:832396. doi: 10.1155/2013/832396. Epub 2013 Jan 16.

Transplantation of Nonexpanded Adipose Stromal Vascular Fraction and Platelet-Rich Plasma for Articular Cartilage Injury Treatment in Mice Model

 Phuc Van Pham ¹, Khanh Hong-Thien Bui ², Dat Quoc Ngo ³, Lam Tan Khuat ¹, Ngoc Kim Phan ¹ Affiliations expandPMID: 27006923 PMCID: PMC4782730 DOI: 10.1155/2013/832396

World J Clin Cases. 2021 Jun 26; 9(18): 4797–4802.

Published online 2021 Jun 26. doi: 10.12998/wjcc.v9.i18.4797PMCID: PMC8223823 PMID: 34222450 

Therapeutic effect of autologous concentrated growth factor on lower-extremity chronic refractory wounds: A case report 

Po Liu, Yang Liu, Chang-Neng Ke, Wei-Shan Li, Yue-Ming Liu, and Shi Xu

International Journal of Implant Dentistry Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) Kazushige Isobe1, Taisuke Watanebe1, Hideo Kawabata1, Yutaka Kitamura1, Toshimitsu Okudera1, Hajime Okudera1, Kohya Uematsu2, Kazuhiro Okuda3, Koh Nakata4, Takaaki Tanaka5 and Tomoyuki Kawase6*of the article of Implant Dentistry (2017) 3:17 DOI 10.1186/s40729-017-0081-7

MICROSCOPY RESEARCH AND TECHNIQUE 74:772–777 (2011) Growth Factors, CD34 Positive Cells, and Fibrin Network Analysis in Concentrated Growth Factors Fraction LUIGI FABRIZIO RODELLA,1* GAIA FAVERO,1 RAMON BONINSEGNA,1 BARBARA BUFFOLI,1MAURO LABANCA,1 GIORGIO SCARI`,2 LUIGI SACCO,3 TIZIANO BATANI,4 AND RITA REZZANI1 1Department of Biomedical Sciences and Biotechnologies, Division of Human Anatomy, University of Brescia, V.le Europa 11, 25123 Brescia, Italy2Department of Biology, University of Milan, Via Celoria 26, 20133 Milano, Italy3Private Practician, Oral Surgeon, Sersale (CZ), Italy 4Silfradent srl, Via G. Di Vittorio n.35/37—47018 S.Sofia (FC), Italy

Biology and Medicine Borsani et al., Biol Med (Aligarh) 2015, 7:5 http://dx.doi.org/10.4172/0974-8369.1000256 Biol Med (Aligarh) Volume 7 • Issue 5 • 1000256 ISSN: 0974-8369 BLM, an open access journal Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Masuki et al. International Journal of Implant Dentistry (2016) 2:19 DOI 10.1186/s40729-016-0052-4 Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF) Hideo Masuki1†, Toshimitsu Okudera1†, Taisuke Watanebe1, Masashi Suzuki1, Kazuhiko Nishiyama1, Hajime Okudera1, Koh Nakata2, Kohya Uematsu4, Chen-Yao Su3 and Tomoyuki Kawase4*

Appl. Sci. 2022 The Growth Factor Release from a Platelet-Rich Plasma Preparation Is Influenced by the Onset of Guttate Psoriasis: A Case Report by Elisa Borsani 1,2,* Barbara Buffoli 1,2 Francesca Bonomini 1,2 Rita Rezzani 1,2 ^1,2andDepartment of Clinical and Experimental Sciences, Division of Anatomy and Physiopathology, University of Brescia, 25123 Brescia, Italy²Interdepartmental University Center of Research “Adaption and Regeneration of Tissues and Organs-(ARTO)”, University of Brescia, 25123 Brescia, Italy Author to whom correspondence should be addressed. Appl. Sci. 2022, 12(14), 7250; https://doi.org/10.3390/app12147250 Published: 19 July 2022

Ann Maxillofac Surg 2020 Jul-Dec;10(2):409-416.doi: 10.4103/ams.ams_154_19.Epub 2020 Dec 23.

Efficacy of Platelet-Rich Plasma and Concentrated Growth Factor in Treating Androgenetic Alopecia - A Retrospective Study Enosh Nirmalkumar Steward ¹, Hiren Patel ¹, Haren Pandya ¹, Hitesh Dewan ¹, Bijal Bhavsar ¹, Urvi Shah ¹, Kartik Dholakia ¹ Affiliations expand PMID: 33708587 PMCID: PMC7943983 DOI: 10.4103/ams.ams_154_19

Clinical observation and evaluation of CGF in the treatment of androgenic alopecia Fangning Zhao MD1 | Lijun Hao MD2 | Xiaolei Jin MD1

Facial Plast Surg Aesthet Med 2021 Jul-Aug;23(4):255-262. doi: 10.1089/fpsam.2020.0288. Epub 2020 Aug 21.

Autologous Concentrated Growth Factors Combined with Topical Minoxidil for the Treatment of Male Androgenetic Alopecia: A Randomized Controlled Clinical Trial  Poh-Ching Tan ¹, Pei-Qi Zhang ¹, Yun Xie ¹, Yi-Ming Gao ¹, Qing-Feng Li ¹, Shuang-Bai Zhou ¹, Qing Liu ¹, Kai Liu ¹ Affiliations expand PMID: 32833539 DOI: 10.1089/fpsam.2020.0288

Clinical observation and evaluation of CGF in the treatment of androgenic alopecia.Zhao F, Hao L, Jin X.J Cosmet Dermatol. 2022 Nov;21(11):5723-5729. doi: 10.1111/jocd.15109. Epub 2022 Jun 4.PMID: 35608516

J Cosmet Dermatol 2022 Nov;21(11):5723-5729. doi: 10.1111/jocd.15109. Epub 2022 Jun 4.Clinical observation and evaluation of CGF in the treatment of androgenic alopecia Fangning Zhao ¹, Lijun Hao ², Xiaolei Jin ¹Affiliations expand PMID: 35608516 DOI: 10.1111/jocd.15109

2021 May;20(5):1421-1426. doi: 10.1111/jocd.13742. Epub 2020 Oct 12.

Injectable Platelet-Rich Fibrin (PRF): The newest biomaterial and its use in various dermatological conditions in our practice: A case series Bansod Shashank ¹, Madke Bhushan ² Affiliations expand PMID: 32996229 DOI: 10.1111/jocd.13742

Indian Dermatol Online J 2021 Nov 25;12(Suppl 1):S31-S40.doi: 10.4103/idoj.idoj_328_21.eCollection 2021 Nov. Platelet-Rich Plasma in Androgenetic Alopecia Aseem Sharma ¹, Kavish Chouhan ², Satish Bhatia ³, Shuken Dashore ⁴ Affiliations expandPMID: 34976879 PMCID: PMC8664169 DOI: 10.4103/idoj.idoj_328_21

J Biol Regul Homeost Agents 2021 Mar-Apr;35(2 Suppl. 1):345-350 doi: 10.23812/21-2supp1-34.Concentrated growth factors gel activated with ozone for facial aesthetics purpose after granuloma removal: a case reportP Pederzoli ¹, A Greco Lucchina ², M Del Fabbro ^3 4, C Mortellaro ²Affiliations expand PMID: 34281331 DOI: 10.23812/21-2supp1-34

Cytokine Growth Factor Rev 2022 Apr;64:84-94. doi: 10.1016/j.cytogfr.2021.11.003.Epub 2021 Dec 2.

Applications of the regenerative capacity of platelets in modern medicine Elżbieta Cecerska-Heryć ¹, Małgorzata Goszka ², Natalia Serwin ², Marta Roszak ², Bartłomiej Grygorcewicz ², Rafał Heryć ³, Barbara Dołęgowska ²Affiliations expand PMID: 34924312DOI: 10.1016/j.cytogfr.2021.11.003

Int J Trichology 2019 May-Jun;11(3):128-131. doi: 10.4103/ijt.ijt_73_18. Injectable-Platelet-Rich Fibrin-Smart Blood with Stem Cells for the Treatment of Alopecia: A Report of Three Patients - Ritika Arora ¹, Sagrika Shukla ² Affiliations expand PMID: 31360042 PMCID: PMC6580805 DOI: 10.4103/ijt.ijt_73_1

Randomized Controlled Trial Plast Reconstr Surg 2023 Aug 1;152(2):281e-292e doi: 10.1097/PRS.0000000000010227. Epub 2023 Jan 24.Autologous Concentrated Growth Factor Increases Skin Thickness and Area during Tissue Expansion: A Randomized Clinical Trial Poh-Ching Tan ¹, Pei-Qi Zhang ¹, Shuang-Bai Zhou ¹, Jizhou He ¹, Jia Qian ¹, Ru-Lin Huang ¹, Zhi-Yue Zhang ¹, Chen Cheng ¹, Qingfeng Li ¹, Yun Xie ¹ Affiliations expand PMID: 36727707 DOI: 10.1097/PRS.0000000000010227

Use of PRP, PRF and CGF in Periodontal Regeneration and Facial Rejuvenation-A Narrative ReviewMijiritsky E, Assaf HD, Peleg O, Shacham M, Cerroni L, Mangani L.Biology (Basel). 2021 Apr 10;10(4):317. doi: 10.3390/biology10040317.PMID: 33920204 Open Med (Wars) 2019 Dec 24;14:959-967. doi: 10.1515/med-2019-0113. eCollection 2019.

CGF Treatment of Leg Ulcers: a Randomized Controlled Trial Bruno Amato ^1 2, Michele Angelo Farina ³, Silvana Campisi ⁴, Marino Ciliberti ⁵, Vincenzo Di Donna ⁶, Anna Florio ⁷, Antonino Grasso ⁸, Rosario Miranda ⁹, Francesco Pompeo ¹⁰, Eleonora Farina ³, Raffaele Serra ^2 11, Roberto Cirocchi ¹², Francesca Calemma ¹, Aldo Rocca ¹³, Rita Compagna ^2 7 Affiliations expand PMID: 31934641PMCID: PMC6947757 DOI: 10.1515/med-2019-0113

J Int Med Res 2020 Oct;48(10):300060520962946.doi: 10.1177/0300060520962946.Therapeutic effect of concentrated growth factor preparation on skin photoaging in a mouse model Rongrong Zhou ^1 2, Miao Wang ³, Xudong Zhang ², Aifen Chen ², Yanghonghong Fei ², Qiming Zhao ⁴, Danjing Guo ¹, Hui Chen ¹, Shusen Zheng ¹

Affiliations expand PMID: 3311531 PMCID: PMC7645418DOI: 10.1177/0300060520962946

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Use of APAG device as per Dr. Gregorio Martinez’s Protocol (April 2019 - Rev. 00)

Lee SB, Lim AR, Rah DK, Kim KS, Min HJ. Modulation of heat shock protein 90 affects TGF-beta-induced collagen synthesis in human dermal fibroblast cells. Tissue Cell. Dec 2016;48(6):616-623.

-- Use of APAG device as per Dr. Gregorio Martinez’

Noble-Daoud Aniss A, Magdy Zaazaa A, Rabie Abdalla Seleh M. Anti-arthritic effects of platelets rich plasma and hyaluronic acid on adjuvant-induced arthrtitis in rats. International Journal of Pharmacology 16(1): 33-46; 2020

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Use of PRP, PRF and CGF in Periodontal Regeneration and Facial Rejuvenation-A Narrative Review Eitan Mijiritsky ^1 2, Haya Drora Assaf ³,Oren Peleg ¹, Maayan Shacham ⁴, Loredana Cerroni ⁵, Luca Mangani ⁵ Affiliationsj molecular sciences article analysis of cgf biomolecules, structure and cell population: characterization of the stemness features of cgf cells and osteogenic potential eleonora stanca 1,*, nadia calabriso 2 , laura giannotti 1, paola nitti 3,4 , fabrizio damiano 1 ,benedetta di chiara stanca 1, maria annunziata carluccio 2 , giuseppe egidio de benedetto 4 ,christian demitri 3, andrea palermo 5, franco ferrante 6 , luisa siculella 1,* and alessio rochira 1,* int. j. mol. sci. 2021, 22, 8867 2 of

biological characterization and in vitro effects of human concentrated growth factor preparation: an innovative approach to tissue regeneration january 2015 biology and medicine 07(05) doi:10.4172/0974-8369.1000256

cgf- clinical studie jan.2021 - yüce mo, adalı e, işık g. the effect of concentrated growth factor (cgf) in the surgical treatment of medication-related osteonecrosis of the jaw (mronj) in osteoporosis patients: a randomized controlled study. clin oral investig. 2021 - jan 3. doi: 10.1007/s00784-020-03766-8.

cgf- clinical studie jan, 2021 - adalı e, yüce mo, günbay t, günbay s. does concentrated growth factor used with allografts in maxillary sinus lifting have adjunctive benefits? j oral maxillofac surg. 2021 jan;79(1):98-108. doi:10.1016/j.joms.2020.07.217.

cgf clinical studie, dec. 2020 - fang h, liu q, cheng t, yang c, wu r, yang z, yang d. innovative use of concentrated growth factors combined with corticosteroids to treat discoid lupus erythematosus alopecia: a case report. j cosmet dermatol. 2020 dec 23. doi: 10.1111/jocd.13904.

cgf- in vitro studie, oct. 2020 rochira a, siculella l, damiano f, palermo a, ferrante f, carluccio ma, calabriso n, giannotti l, stanca e. concentrated growth factors (cgf) induce osteogenic differentiation in human bone marrow stem cells. biology (basel). 2020 oct 30;9(11):370. doi: 10.3390/biology9110370

cgf clinical studie, aug. 2020 tan pc, zhang pq, xie y, gao ym, li qf, zhou sb, liu q, liu k. autologous concentrated growth factors combined with topical minoxidil for the treatment of male androgenetic alopecia: a randomized controlled clinical trial. facial plast surg aesthet med. 2020 aug 21. doi:10.1089/fpsam.2020.0288.

cgf in vitro studie, aug 2020- wei z, liu s, chen z, huang x. comparison of the effect on proliferation of osteoblasts mc3t3-e1 between the concentrated growth factor extract and the platelet-rich fibrin extract. zhong nan da xue xue bao yi xue ban. 2020 aug 28;45(8):901-908. english, chinese.doi: 10.11817/j.issn.1672-7347.2020.190037. and anti-magnetic r

cgf in vitro studie, jul. 2020 - zhang m, zhang t, tang y, ren g, zhang y, ren x. concentrated growth factor inhibits uva-induced photoaging in human dermal fibroblasts via the mapk/ap-1 pathway. biosci rep. 2020 jul 31;40(7):bsr20193566. doi: 10.1042/bsr20193566.

cgf clinical studie, may. 2020 - kamal a, salman b, abdul razak nh, qabbani aa, samsudin ar. the efficacy of concentrated growth factor in the healing of alveolar osteitis: a clinical study. int j dent. 2020a may 12;2020:9038629. doi: 10.1155/2020/9038629.

cgf clinical studie, apr. 2020 - çankaya zt, ünsal b, gürbüz s, bakirarar b, tamam e. efficiency of concentrated growth factor in the surgical treatment of multiple adjacent papillary losses: a randomized, controlled, examiner-blinded clinical trial using cad/cam. int j periodontics restorative dent. 2020 mar/apr;40(2):e73-e83. doi: 10.11607/prd.4359. richiesto pdf

clinical studie , apr. 2020- brignardello-petersen r. concentrated growth factor seems to have benefits in outcomes after extraction of partially impacted third molars. j am dent assoc. 2020 apr;151(4):e29. doi: 10.1016/j.adaj.2019.10.018. richiesto pdf

clinical studie, apr. 2020 -zhao qm, gao j, huang xx, chen xp, wang x. concentrated growth factors extracted from blood plasma used to repair nasal septal mucosal defect after rhinoplasty. aesthetic plast surg. 2020 apr;44(2):511-516. doi: 10.1007/s00266-019-01474-7.

clinical studie, mar. 2020 - sun jl, wang jj, cui zj, meng qn, liu xj, wang x, yu zg. [clinical effects of concentrated growth factor combined with plasma albumin gel in treating facial depressed scar]. zhonghua shao shang za zhi. 2020 mar 20;36(3):210-218. chinese. doi: 10.3760/cma.j.cn501120-20190930-00389.

clinical studie, feb. 2020 - kao ch. use of concentrate growth factors gel or membrane in chronic wound healing: description of 18 cases. int wound j. 2020 feb;17(1):158-166. doi: 10.1111/iwj.13250.

clinical studie, feb. 2020- torul d, omezli mm, kahveci k. evaluation of the effects of concentrated growth factors or advanced platelet rich-fibrin on postoperative pain, edema, and trismus following lower third molar removal: a randomized controlled clinical trial. j stomatol oral maxillofac surg. 2020 dec;121(6):646-651. doi: 10.1016/j.jormas.2020.02.004.

in vitro studie, jan 2020 - borsani e, buffoli b, bonazza v, brunelli g, monini l, inchingolo f, ballini a, rezzani r, rodella lf. in vitro effects of concentrated growth factors (cgf) on human sh-sy5y neuronal cells. eur rev med pharmacol sci. 2020 jan;24(1):304-314.

clinical studie, dec. 2019 - amato b, farina ma, campisi s, ciliberti m, donna vd, florio a, grasso a, miranda r, pompeo f, farina e, serra r, cirocchi r, calemma f, rocca a, compagna r. cgf treatment of leg ulcers: a randomized controlled trial. open med (wars). 2019 dec 24;14:959-967. doi: 10.1515/med-2019-0113.

clinical studie, dec. 2019 - krishnakumar d, mahendra j, ari g, perumalsamy r. a clinical and histological evaluation of platelet-rich fibrin and cgf for root coverage procedure using coronally advanced flap: a split-mouth design. indian j dent res. 2019 nov-dec;30(6):970-974. doi: 10.4103/ijdr.ijdr_16_18.

in vitro studie, may 2019- chen j, jiao d, zhang m, zhong s, zhang t, ren x, ren g. concentrated growth factors can inhibit photoaging damage induced by ultraviolet a (uva) on the human dermal fibroblasts in vitro. med sci monit. 2019 may 20;25:3739-3749.doi: 10.12659/msm.913967.

in vitro studie, feb. 2019 - li x, yang h, zhang z, yan z, lv h, zhang y, wu b. concentrated growth factor exudate enhances the proliferation of human periodontal ligament cells in the presence of tnf‑α. mol med rep. 2019 feb;19(2):943-950. doi: 10.3892/mmr.2018.9714.

evidence for contamination of silica microparticlesin advanced platelet-rich fibrin matrices prepared using silica-coated plastic tubes * correspondence: kawase@dent.niigata-u.ac.jp; tel.: +81-25-262-7559 received: 20 may 2019; accepted: 18 june 2019; published: 19 june 2019

blood tubes literature:received: 4 may 2016 | revised: 29 july 2016 | accepted: 23 august 2016doi 10.1002/jemt.22772 how the different material and shape of the blood collection tube influences the concentrated growth factors production veronica bonazza1 | elisa borsani1,2 | barbara buffoli1,2 | stefania castrezzati1 |rita rezzani1,2 | luigi fabrizio rodella1,2 article in odontology · january 2020 doi: 10.1007/s10266-020-00486-zacute cytotoxic effects of silica microparticles used for coating of plastic blood-collection tubes on human periosteal cells